To evaluate growth on solid media, in all cases -with the exception of the metE mutant, which only grew in the presence of B12 and, therefore, plating with and without B12 was performed from liquid cultures supplemented with B12- the liquid cultures grown without B12 were plated in solid 7H10-ADC plates supplemented with or without AdoCbl (10 g/mL) and/or L-Met (25 g/mL)
Patients following such a diet, should be advised to take oral vitamin B12 regularly
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Sealed vials expire on the printed date, usually 2 to 3 years from manufacture
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If you cancel the application If the treatment is interrupted, you endanger the success of the treatment