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Collectively, these in vitro data provide direct mechanistic evidence that exerkine pathways can modulate cell viability, apoptosis, barrier function, ROS generation and inflammatory mediators in retinal cells relevant to DR ( Figure 3 )

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reported that KGN cells treated with DHEA exhibited ferroptosis characterized by decreased viability, inhibited GPX4 and SLC7A11 expression, increased ACSL4 expression, increased MDA levels and ROS accumulation, and increased lipid peroxidation