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The staining can be stopped, when the OD value of the darkest color at 620nm is between 0.8 - 0.9
It helps keep cells running smoothly
Its important to note that, when youre packing your own columns, this is *not* the volume of stuff you pipet out of the bottle instead thats typically a slurry of resin & storage solution (often at a 50% or 75% mixture)
Sleep architecture (slow-wave sleep)
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