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Methods We searched four electronic databases (PubMed, Scopus, Web of Science, and Cochrane CENTRAL Register of Controlled Trials)

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Experimental Consistency: Using the same sterile solvent across multiple protein preparations improves reproducibility and reduces unwanted microbial variables

Bearing in mind an estimated 8-10% prevalence of vitamin B12 deficiency among the population of this age group, an initial sample of 3556 patients will need to be recruited

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Do not store it at room temperature, and do not freeze it unless you have a specific long-term storage protocol, as repeated freeze-thaw cycles can also degrade the compound