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doi: 10.1177/0961203316629558 562 ShanksLJasonLAEvansMBrownA

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Briefly, different concentrations of TLC-2716 or TLC-6665 were incubated with an assay buffer (Tris/HCl buffer (pH 6.8) containing 240 mM KCl, 1 g l 1 bovine serum albumin and 0.002% Triton X-100) containing 530 g ml 1 recombinant GST-tagged LXR/LXR LBD, 125 pg l 1 GST-Tb cryptate (CisBio), 400 nM N-terminally biotinylated NCOA3 (coactivator) peptide, 1 M 24( S )-25-epoxycholesterol (LXR agonist, only in the NCOA3 assay) and 2.5 ng l 1 Streptavidin-XL665 (CisBio) for 1 h at 4 C, following which fluorescence was measured in a VictorX4 multiplate reader (PerkinElmer Life Science) using 340 nm as excitation and 615 and 665 nm as emission wavelengths