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Concurrent alcohol intake lowers these thresholds significantly

CIS is widely used in many types of tumors, such as lung, ovarian, breast, bladder, and testicular cancers, but drug resistance and multi-organ toxicity limit its practical use (58)

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Epub 2012 Feb 28

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For colony formation assay, cells were cultured in 24-well tissue culture plates for a week to form colonies before being fixed with 10% neutral formalin, stained with 0.5% crystal violet solution, and having the dye extracted by adding 10% acetic acid