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Cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS, L-glutamine, penicillin, and streptomycin at 37 C in 5% CO 2

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(NOTE: The results were replicated in a separate study using a different NNMT inhibitor, also observing the normalization of tolerance to glucose and increased insulin sensitivity) Already, were seeing some strong interest in using 5-Amino 1MQ to treat obesity and other metabolic diseases in US veterans