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When influence of Fab 3F52 on GLP-1R ligands (native GLP-1, BMS21, compound 2) was examined, ligands plus Fab 3F52 were added simultaneously to the cells (50 l/well)
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pH was adjusted to 7 with 1M NaOH (Merck, Italy)
Pinch a fold of skin approximately 1 inch thick at the injection site (abdomen preferred, at least 2 inches from navel) [10]
GLP-1 binding to GLP-1R promotes -cell glucose metabolism through mTOR-dependent HIF-1 activation
Credit: Holst 2024, figure 1.2.4
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