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An additional open question is whether propagating ferroptotic injury contains reversible intermediate states before reaching tissue-scale collapse or whether spread proceeds as a strictly irreversible process once initiated

All pretreatments increased binding of autologous IgG as well as of purified anti-band 3 NAbs and binding was inhibited by purified band 3 protein or by restoration of the protein SH groups (Beppu et al., Oxidation or a treatment with a clustering agent and cross-linking of the generated clusters evidently simulates the prerequisites for clearance of RBC, but does not explain how band 3 clusters are formed during in vivo aging