The threshold for this year is $821 million
A further mechanistic consideration is whether the pomegranate extract may have influenced plasma TMAO concentrations partly through its effect on the absorption of L-carnitine in the small intestine, rather than solely through inhibition of microbial TMA production
More elegantly, the peptide itself can be redesigned for stability unnatural amino acids (like Aib), cyclization, D-amino acid substitutions, and PEGylation all make the molecule harder for proteases to cleave and can extend half-life
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Protein bands were visualized with an ECL detection kit, and the intensity of protein bands was quantitated by Image Lab 6.1 software (Bio-Rad Laboratories, Hercules, CA)