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Data processing All data obtained from the four assays with the two systems were processed with software (Progenesis QI 3.0.3.0, Nonlinear Dynamic, Newcastle, UK) for peak picking, alignment, and normalization to produce peak intensities for retention time ( t R ) and mass-to-charge ratio ( m/z ) data pairs

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( C ) Seahorse XFe24 traces of glucose oxidation, measured as the OCR by sequential injection of 20 mM glucose, 1 M oligomycin, 1 M FCCP [carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone], 1 M rotenone, and 2 M antimycin A into caSMCs cultured in normal (5.5 mmol/L) glucose media

You can usually return to your daily activities right after the treatment