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In a 6 multi-well plate, 1.5 10 5 cells were plated and transfected with 900 ng of pHIV-dTomato and 100 ng of pIRES2-EGFP (Clontech #6029-1, Mountain View, CA, USA), a transfection efficiency control, using Lipofectamine 3000 Transfection Reagent (Invitrogen, Life Technologies)

Remarkably, the upregulation is observed after initial decrease in the five thiamine/ThDP-dependent proteins 1 month after the bypass (Figures 4A, 5A)

Certain nutrients trigger more GLP-1 release than others

Two-tailed paired t-test (ns: not significant, p = 0.2213)

GLP-1 medications have been shown to reduce alcohol cravings in some studies

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