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Identification of the exact mechanism for the lethal effect of silencing the enzyme requires further experiments, as do the effects of inhibitors, which are not necessarily solely due to loss of enzyme activity, because the inhibited enzyme may also have a gain of function leading to cell death [72]

The article calls that distinction crucial for both safety and value, and says pricing depends on pharmacy licensing, raw material sourcing, and regulatory compliance

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The sequence they appear in determines what they do