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doi: 10.1016/j.ccell.2022.02.003
Repeatedly opening and closing a vial introduces fresh oxygen, providing the fuel for oxidative degradation
We used ionized calcium-binding adapter molecule 1 (IBA1), a common marker for microglia, for immunostaining
Principle: Endotoxins are lipopolysaccharides (LPS) from the outer membrane of Gram-negative bacteria
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Diluent pH acting alone is the least likely explanation, because bacteriostatic water is unbuffered and the peptides counterion dominates the final pH
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