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This chart may be useful as an overview: What Are The Symptoms of Vitamin B12 Deficiency

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A review of individual studies found that people with lower serum vitamin B12 levels are at a higher risk for depression

B12 shots are very safe with minimal side effects

The study found no impact on folate levels, but reported a 5% reduction in serum B12 levels when women went from 0 to 30 g of alcohol a day