However, anorexia, GI surgery and malabsorption, reduced intake of iodized supplements, and the area of origin (as soil levels of iodine are very low in mountainous regions) may represent risk factors for hypothyroidism
Functional indicators of oxidative stress included relative levels of 3-chlorotyrosine (3-CT), an established biomarker of a chronic inflammatory response, and aconitase activity, a biomarker of mitochondrial superoxide production
Yamane et al., 2010)
Chronic stress can lead to glutathione depletion
Furthermore, it has been reported that roGFP1 under conditions of intense illumination undergoes an irreversible photoswitch reaction that would foster the anionic form of the chromophore and thus may artificially report reducing conditions even though the local environment of the probe is oxidizing (Schwarzlnder et al., 2008)
Glutathione in high amounts is not easily tolerated by everyone