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Following three washing steps with PBS, cells were permeabilized with 0.1% Triton for 20 min and blocked with 2% BSA in PBS-Tween (0.5%) at room temperature for at least 1 h

Based on a strong tradition in evidence-based treatments combined with a cost effective healthcare system many age related diseases are coming to market earlier in a chronic disease guideline framework

The sections were then dehydrated, cleared, and eventually mounted in entellane (Merck Co., Germany)

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The weight loss achieved can reduce the risk of other metabolic problems associated with being overweight