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This is one of the most common questions in the GHK-Cu space, and the answer is more nuanced than either camp usually admits

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Existence of genetic variants of thiamine-dependent proteins, insufficient molecular identification of these proteins, as well as the age- and sex-dependent differences in thiamine metabolism (30), may contribute to the observed variety of clinical manifestations of thiamine deficiency (31, 32)

Lemieux, G

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Cells were cultured in a humified 5% CO 2 incubator at 37 C