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FlpInCHO wildtype and mutant human GLP-1R cells were seeded at a density of 25 10 4 cells/well into 24-well culture plates and incubated overnight at 37 C in 5% CO 2 , washed three times in 1 PBS and fixed with 3.7% paraformaldehyde (PFA) at 4 C for 15 min

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From pathophysiology to practice: addressing oxidative stress and sperm DNA fragmentation in Varicocele-affected subfertile men

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