Sircol assay on conditioned medium) 29 6% while preserving the cell viability and signalling-independent functions of TGF-1 Smad2/3 signalling
Kido J, Haberle J, Sugawara K, Tanaka T, Nagao M, Sawada T, et al
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Carbonyl Content Analysis The carbonyl content was determined by absorbance at 370 nm, and the total carbonyl content was estimated by the difference in absorbance between DNPH-treated and HCl-treated samples, as described by (Reznick and Packer 1994)

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Activating the immune system , leading to inflammatory responses throughout the body