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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

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Samples were reconstituted in 3060 l 50% acetonitrile, followed by vortexing for 30 sec, and then centrifuged at 10,000 rpm for 10 min at 4 C

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Ive combed through the literature extensively, and while the healing data is impressive, the testosterone connection simply isnt there in the direct mechanistic sense