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The inflammation can be exacerbated by: Hygiene practices: Inadequate cleaning after bowel movements can lead to irritation, while excessive cleaning can strip the skin of its natural oils, causing further discomfort

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Membranes were then incubated with the following primary antibodies diluted in the blocking solution: anti-AKT (#9272, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-AKT (Thr308) (#2965, 1:1000, Cell Signaling), anti-BDNF (sc-546, 1:500, Santa Cruz Biotechnology), anti-ERK1/2 (#9102, 1:1000, Cell Signaling), anti-phospho-ERK1/2 (Thr202/Tyr204) (#4370, 1:1000, Cell Signaling), anti-TrkB (#4603, 1:1000, Cell Signaling), anti-pTrkB (Tyr516) (#4619, 1:1000, Cell Signaling), anti--actin (#4970, 1:1000, Cell Signaling), anti-STAT3 (#9139, 1:1000, Cell Signaling), anti-p-STAT3 (#9145, 1:1000, Cell Signaling)

But as it affects different systems in the body, it might also have unintended effects, such as influencing body temperature regulation

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