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400 nL of the protein/DNA complex was mixed with 200 nL of mother liquor (68 mM Na cacodylate, pH 6.5, 20.4% (w/v) PEG8000, 136 mM ammonium sulfate, 12% (v/v) glycerol)
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Although most of the point mutants exhibited only slightly reduced activity, both of double and triple mutants showed significant deficiency in inducing c-MET activation (Fig
No, because B12 is water-soluble
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